Efficient human fetal liver cell isolation protocol based on vascular perfusion for liver cell–based therapy and case report on cell transplantation
✍ Scribed by Bruno Gridelli; Giovanni Vizzini; Giada Pietrosi; Angelo Luca; Marco Spada; Salvatore Gruttadauria; Davide Cintorino; Giandomenico Amico; Cinzia Chinnici; Toshio Miki; Eva Schmelzer; Pier Giulio Conaldi; Fabio Triolo; Jörg C. Gerlach
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 881 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1527-6465
- DOI
- 10.1002/lt.22322
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✦ Synopsis
Although hepatic cell transplantation (CT) holds the promise of bridging patients with end-stage chronic liver failure to whole liver transplantation, suitable cell populations are under debate. In addition to hepatic cells, mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) are being considered as alternative cell sources for initial clinical cell work. Fetal liver (FL) tissue contains potential progenitors for all these cell lineages. Based on the collagenase incubation of tissue fragments, traditional isolation techniques yield only a fraction of the number of available cells. We report a 5-step method in which a portal vein in situ perfusion technique is used for tissue from the late second trimester. This method results in the high viabilities known for adult liver vascular perfusion, addresses the low cell yields of conventional digestion methods, and reduces the exposure of the tissue to collagenase 4-fold. We used donated tissue from gestational weeks 18 to 22,