The commercial application of lipases as biocatalysts for organic synthesis requires simple but efficient methods to immobilize the enzyme, yielding highly stable and active biocatalysts which are easy to recover. In this study, we present a novel method to achieve lipase immobilization by entrapmen
✦ LIBER ✦
Efficient Heterogeneous Biocatalysts by Entrapment of Lipases in Hydrophobic Sol–Gel Materials
✍ Scribed by Prof. Dr. Manfred T. Reetz; Dipl.-Biochem. Albin Zonta; Dr. Jörg Simpelkamp
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 564 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0044-8249
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## Abstract **BACKGROUND:** Sol–gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol–gel coating of lipase in supercritical carbon dioxide (SC‐CO~2~) is proposed. **RESULTS:** Crude lipase powder (__Rhizopus oryzae__) coated with hydrophobic silicates, deriv