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Efficient FLP recombination in mouse ES cells and oocytes

✍ Scribed by Julia Schaft; Ruth Ashery-Padan; Frank van der Hoeven; Peter Gruss; A. Francis Stewart

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
143 KB
Volume
31
Category
Article
ISSN
1526-954X

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✦ Synopsis

Abstract

Summary: We report an improved vector, pCAGGS‐FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS‐FLPe into oocytes, about one‐third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS‐FLPe presents two practical options for removal of FRT cassettes in mice. genesis 31:6–10, 2001. © 2001 Wiley‐Liss, Inc.

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