The complete (encoding 119 aminon acids, aa) or partial (encoding the N-terminal 90 aa) preS1 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathione-S-transferase (GST) gene and expressed at 37 Β°C under the control of the inducible tac promoter in E. coli. The results showed t
Efficient expression and characterization of hepatitis B virus preS2 antigen inEscherichia coli
β Scribed by Young Jun Kang; Young Shin Kim; Hyangsuk Hur; Hee Sun Kim; Hyo Jeong Hong
- Book ID
- 110225202
- Publisher
- Springer Netherlands
- Year
- 1999
- Tongue
- English
- Weight
- 84 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0141-5492
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## Abstract The infectious particles of hepatitis B virus (HBV) contain 3 related surface antigens, i.e., small, medium, and large, all of which are encoded by one large open reading frame with multiple initiation codons. The large surface antigen (LβAg) contains preS1, preS2, and S regions while b
## Abstract We constructed a recombinant plasmid that can express the entire preβS2 sequence of hepatitis B surface antigen (HBsAg) as a fusion protein in __E. coli__. The hybrid protein, which comprises the bacterial TrpLE protein and the preβS2 sequence, was the prominent protein that was found i