Efficient elutriation of monocytes within a closed system (Elutra™) for clinical-scale generation of dendritic cells
✍ Scribed by Thomas G. Berger; Erwin Strasser; Richard Smith; Curt Carste; Beatrice Schuler-Thurner; Eckhart Kaempgen; Gerold Schuler
- Book ID
- 113716951
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 490 KB
- Volume
- 298
- Category
- Article
- ISSN
- 0022-1759
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Dendritic cells (DC) used for clinical trials should be processed on a large scale conforming to current good manufacturing practice (cGMP) guidelines. The aim of this study was to develop a protocol for clinical grade generation of immature DC in a closed-system. Aphereses were performed with the C
## Abstract Monocytes are a common source for generating dendritic cells (DCs). The aim of the present study was to evaluate the efficiency of a platform for monocyte collection and enrichment in a clinical setting. The platform was based on the combination of two semiautomated devices; the Cobe Sp