Efficient detection of CαH-HN correlations in proteins using gradient-enhanced 15N HMQC-TOCSY

HNCCH-TOCSY, a triple resonance experime

HNCCH-TOCSY, a triple resonance experiment for the correlation of backbone13Cαand15N resonances with aliphatic side-chain proton resonances and for measuring vicinal13CO,1Hβcoupling constants in proteins

✍ Rüdiger Weisemann; Frank Löhr; Heinz Rüterjans 📂 Article 📅 1994 🏛 Springer Netherlands 🌐 English ⚖ 489 KB

A 3D triple resonance experiment has been designed to provide intraresidual and sequential correlations between amide nitrogens and c~-carbons in uniformly 13C/15N-labeled proteins. In-phase 13C~ magnetization is transferred to the aliphatic side-chain protons via the side-chain carbons using a CC-T