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Efficient cloning of a mutant adenylate-kinase-encoding gene from Escherichia coli

โœ Scribed by Liang, Peng; Glaser, Michael

Book ID
122382794
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
911 KB
Volume
80
Category
Article
ISSN
0378-1119

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We have cloned genes encoding RNase H from Escherichia coli rnh mutants, Salmonella typhimurium and Saccharomyces cerevisiae. Selection was accomplished by suppression of the temperature-sensitive growth phenotype of Escherichia coli strains containing the rnh-339::cat and either recB270 (Ts) or rec