Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts
β Scribed by Michael A. Von Tersch; Helen Loidl Robbins
- Book ID
- 109316693
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 254 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0378-1097
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DNA from Clostridium acetobutylicum ABKn8 was partially digested with Sau3A and the fragments obtained were inserted into the unique BamHI site of the cloning vector pHV33. The recombinant plasmids were used to transform Escherichia coli HB101 with selection for ampicillin resistance. A collection o