Effects on in vitro tumor growth of murine macrophages isolated from sarcoma lines differing in immunogenicity and metastasizing capacity
✍ Scribed by Alberto Mantovani
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- French
- Weight
- 497 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Macrophages were isolated from the strongly immunogenic, non‐metastasizing FS6 sarcoma transplanted in syngeneic C57BL/6 mice and from a weakly immunogenic, spontaneously metastasizing line (mFS6) of the same tumor. Macrophages isolated from the FS6 sarcoma non‐specifically inhibited growth and DNA synthesis of tumor cells in vitro. After culture FS6 macrophages rapidly lost their cytotoxic activity and became capable of non‐specifically stimulating tumor growth in vitro. Macrophages obtained from the mFS6 tumor non‐specifically enhanced the proliferative activity of tumor target cells and this capacity was maintained when the macrophages were cultured.