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Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration ofPhalaenopsis

✍ Scribed by Yongqin Chen; Chitrapan Piluek

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 581 KB
Volume: 16
Category: Article
ISSN: 0167-6903
DOI: 10.1007/bf00040513

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✦ Synopsis

Adventitious bud formation from the vegetative buds of the flower stalks of Phalaenopsis occurred on Vacin and Went medium with 15% coconut water and 5 to 40 pM thidiazuron (TDZ) or 40 pM N6-benzylaminopurine. The highest efficiency of induction was achieved with 5 or 10 pM TDZ. Adventitious buds developed into shoots on VWC medium. TDZ was more effective than BAP in stimulating the axillary buds of intact shoots to develop. Regenerated shoots rooted after about two months of culture on VWC medium with 1% sucrose. Shoot tips excised from the regenerated shoots initiated protocorm-like bodies after two months of culture on VWC medium.

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