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Effects of the purified IGF-I complex on the capacitation and acrosome reaction of rabbit spermatozoa

✍ Scribed by Minelli, Alba ;Liguori, Lavinia ;Collodel, Giulia ;Lattaioli, Paolo ;Castellini, Cesare

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 92 KB
Volume: 290
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1061

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✦ Synopsis

Abstract

A protein complex containing IGF‐I, purified from rabbit seminal plasma, was used to investigate its effects on the capacitation and acrosome reaction of rabbit spermatozoa. Uncapacitated sperm (Pattern F), capacitated sperm (Pattern B), and acrosome‐reacted sperm (Pattern AR) were determined by CTC staining, and the results were validated by PSA‐staining. The addition of the IGF‐I complex to the capacitative medium directed the spermatozoa to spontaneous acrosome reaction. On the other hand, IGF‐I complex, added to capacitated spermatozoa, acted as inducer of the acrosome reaction. Results of IVF experiments showed high rates of fertilization with capacitated spermatozoa, acrosome‐reacted by either A23187 or IGF I complex, whereas significantly lower rates were obtained with spermatozoa capacitated in the presence of IGF‐I complex. J. Exp. Zool. 290:311–317, 2001. © 2001 Wiley‐Liss, Inc.

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