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Effects of the flavonoid biochanin A on gene expression in primary human hepatocytes and human intestinal cells

✍ Scribed by Young Jin Moon; Shuzhong Zhang; Daniel A. Brazeau; Marilyn E. Morris

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
391 KB
Volume
51
Category
Article
ISSN
1613-4125

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✦ Synopsis

Abstract

Biochanin A (BCA), a phytoestrogen present in plant food and herbal products, has been reported to have cancer‐preventive effects that may be mediated, in part, through effects on carcinogen metabolism. Our objective was to examine the effect of BCA on gene expression for drug‐metabolizing enzymes and transporters in human hepatocytes. Cells were exposed to 20 μM of BCA for 5 days. Gene expression was assessed by a 96‐gene human drug metabolism enzyme microarray. There were seven genes that were significantly up‐regulated, namely cytochrome P‐450 (CYP) 2A6, CYP2B6, CYP2C9, CYP2F1, multidrug resistance gene (MDR1), thromboxane A synthase 1 (TBXAS1), and SULT1A2 (sulfotransferase). Up‐regulation of MDR1, which encodes for P‐glycoprotein, was confirmed using real‐time RT‐PCR and Western analysis in hepatocytes as well as in human colon adenocarcinoma cell line (LS‐180) and the induction was dose‐dependent. BCA treatment up‐regulated genes mainly in the CYP2 family. This induction can influence the metabolism of xenobiotics, producing effects of pharmacological and toxicological importance.

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