✦ LIBER ✦
Effects of temperature, darkness and gelling agent on long-term storage of in vitro shoot cultures of Australian woody plant species
✍ Scribed by Richard R. Williams; Acram M. Taji
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 292 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Storage of in vitro shoot cultures under reduced temperatures was investigated for eight Australian native woody plant species. Survival of multiple-shoot cultures for up to 12 months was best at 10 °C in the light. Darkness increased the extent of vitrification and lower temperatures reduced survival. Acclimation at an intermediate temperature following storage at 10 °C was sometimes beneficial. Survival was greater on Gelrite-based medium compared with agar, particularly at 22 °C.