Effects of taurine on cell morphology and expression of low-affinity GABA receptors in cultured cerebellar granule cells
✍ Scribed by Jens H. Abraham; Arne Schousboe
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 875 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0364-3190
No coin nor oath required. For personal study only.
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Taurine uptake in cultured cerebellar granule cells and astrocytes consisted of a saturable high-affinity component and nonsaturable diffusion. The transport constant (Km) was significantly lower and the maximal velocity (V) higher in granule cells than in astrocytes. The uptakes were strictly sodiu
## Abstract A glycoprotein that induces γ‐aminobutyric acid (GABA) carriers in cultured cerebellar astrocytes was isolated and purified from conditioned media from cultured cerebellar granule cells by anion exchange chromatography, affinity chromatography, and gel filtration. Following gel filtrati
## Abstract GABA~A~ agonist‐induced formation of low‐affinity GABA~A~ receptors in cultured cerebellar granule cells was studied in the presence or absence of α‐difluoromethylornithine (DFMO), a blocker of polyamine formation. High‐ and low‐affinity GABA~A~ receptors were monitored by Scatchard ana