Effects of sublethal copper exposure on muscle energy metabolism of common carp, measured by 31P-nuclear magnetic resonance spectroscopy

✍ Scribed by Gudrun De Boeck; Robert Borger; Annemie Van der Linden; Ronny Blust

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 152 KB
• Volume: 16
• Category: Article
• ISSN: 0730-7268
• DOI: 10.1002/etc.5620160411

✦ Synopsis

The effects of shock and subchronic exposure (1 week) to 0.36 Ϯ 0.08 and 1.31 Ϯ 0.22 M of copper on the energy metabolism of common carp (Cyprinus carpio) were studied by means of in vivo 31 P-nuclear magnetic resonance spectroscopy ( 31 P-NMRS). During the experiments, fish were submitted to an additional hypoxic challenge and recovery from this challenge was followed for 6 hs. During all experiments adenosine triphosphate (ATP) levels remained stable. Under control conditions, levels of phosphocreatine (P Cr ) and inorganic phosphate (P i ) recovered rapidly after the hypoxic challenge; however, full recovery was not observed after shock copper exposure. Also, intracellular pH (pH i ) did not recover from the hypoxic challenge after shock exposure. After 1 week of exposure the fish had clearly developed an increased tolerance to copper. At both copper concentrations, P Cr and P i levels returned to resting levels after the hypoxic challenge, but at the highest copper concentration P Cr to P i ratios were significantly lower than P Cr to P i ratios in the control group and levels of P Cr and P i were very unstable. At the high copper concentration, pH i was clearly decreased compared to the control group even before the hypoxic challenge.