Effects of serine protease inhibitors on oxyradical burst from phagocytizing neutrophils—analysis by chemiluminescence counting and its microscopic imaging
✍ Scribed by Suematsu, Makoto ;Houzawa, Shigenari ;Miura, Soichiro ;Nagata, Hiroshi ;Kitahora, Tetsuji ;Morishita, Tetsuo ;Oshio, Chikara ;Tsuchiya, Masaharu
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 330 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
Reaction difference of oxyradical generation and luminol-dependent photoemission of zymosan-and phorbol ester-treated neutrophils were investigated using a conventional photomultiplier and ultrasensitive photonic imaging technique. Zymosan-treated cells released a concentrated photonic burst corresponding t o the cellular distribution. In contrast, phorbol ester-treated cells produced a negligible level of photoemission, and the additional application of CaZ+ ionophore enhanced the photonic burst, which was gradually spread out into extracellular space. Serine protease inhibitors did not attenuate PMA-induced chemiluminescence but did attenuate zymosan-induced chemiluminescence. This suggests the involvement of serine protease in the respiratory burst of phagocytizing neutrophils.