𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of retinoic acid and dimethylsulfoxide on the morphogenesis of the sea urchin embryo

✍ Scribed by Serafina Sciarrino; Valeria Matranga

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
435 KB
Volume
19
Category
Article
ISSN
1065-6995

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Sea urchin embryos of the species Paracentrotus lividus were treated continuously with different concentrations of all‐trans retinoic acid (RA) or dimethylsulfoxide (DMSO) at different developmental stages. A delay in embryonic development was observed when embryos were cultured in the presence of 2x10^−5^ M RA, between 1 and 12 hours of development. Hence, at 48 hours of development, while control embryos had reached the pluteus stage, RA‐treated embryos were at the prism stage. At 72 hours of development RA‐treated embryos recovered and continued normal development reaching the pluteus stage. No effect was observed when treatment was performed before 1 hour or after 12 hours of developmet. DMSO treatment had no effect on normal sea urchin embryo development, although we observed that pigment cells, clearly visible at the pluteus stage, become visible earlier with respect to control embryos. This report confirms the advantages that the sea urchin embryo offers for the study of problems in cellular and developmental biology.

📜 SIMILAR VOLUMES

Effect of reduced protein synthesis on t
Effect of reduced protein synthesis on the cell cycle in sea urchin embryos
✍ François Dubé 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 827 KB

We have reinvestigated the existence of cyclical fluctuations of protein synthesis and have examined the effects of reducing it in early embryos of the purple sea urchin, Strongylocentrotus purpuratus. The results show that protein synthesis increases linearly during the first 45-60 minutes after fe

Structure–activity relationship for brom
Structure–activity relationship for bromoindole carbaldehydes: Effects on the sea urchin embryo cell cycle
✍ Isabelle Moubax; Nathalie Bontemps-Subielos; Bernard Banaigs; Georges Combaut; P 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 211 KB

## Abstract Natural derivatives of indole‐3‐carbaldehyde were isolated from the tropical marine ascidian __Stomoza murrayi.__ A series of 13 derivatives, three natural and 10 synthetic (brominated and N‐methylated), were examined for their effects on cell division of sea urchin eggs. These derivati

The effect of cyclophosphamide on the mo
The effect of cyclophosphamide on the morphogenesis of the cerebellum in chick embryos
✍ Singh, Shamer ;Sanyal, A. K. ;Kar, A. K. 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 857 KB

## Abstract The morphogenesis of cerebellum has been studied in 6–19 day old chick embryos after a single injection of 0.05 mg of cyclophosphamide into the yolk sacs on the fifth day of incubation. Besides degeneration of Purkinje cells, rate of development was markedly retarded as evidenced by lat

Biochemical studies of the hatching proc
Biochemical studies of the hatching process in sea urchin embryos. I. Effects of protease inhibitors
✍ Hoshi, Motonori ;Moriya, Tsuneo ;Aoyagi, Takaaki ;Umezawa, Hamao 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 417 KB

## Abstract Effects of 12 protease inhibitors both on the hatching process and on the activity of the hatching enzyme of the sea urchins, __Hemicentrotus pulcherrimus__ and __Strongylocentrotus intermedius__, were studied. Synthetic inhibitors, N‐tosyl‐L‐phenylalanyl‐chloromethane (often called tos