Effects of resveratrol on H2O2-induced apoptosis and expression of SIRTs in H9c2 cells

✍ Scribed by Wei Yu; Yu-Cai Fu; Xiao-Hui Zhou; Chun-Juan Chen; Xin Wang; Rui-Bo Lin; Wei Wang

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 241 KB
• Volume: 107
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22169

✦ Synopsis

Abstract

Resveratrol, a polyphenol found in fruits, has been demonstrated to activate Sir2. Though many studies have demonstrated that resveratrol can activate SIRT1, whether it has effect on other sirtuins (SIRT2–7) are unknown. The present study shows that exposure of H9c2 cells to 50 µM H~2~O~2~ for 6 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry (FCM), but pretreatment of resveratrol (20 µM) eliminated H~2~O~2~‐induced apoptosis. Resveratrol also prevented H~2~O~2~‐induced caspase‐3 activation. Exposure of cells to resveratrol caused rapid activation of SIRT1,3,4,7. Sirtuin inhibitor, nicotinamide (20 mM) attenuated resveratrol's inhibitory effect on cell apoptosis and caspase‐3 activity. These results suggest that resveratrol protects cardiomyocytes from H~2~O~2~‐induced apoptosis by activating SIRT1,3,4,7. J. Cell. Biochem. 107: 741–747, 2009. © 2009 Wiley‐Liss, Inc.