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Effects of Relative Band Intensity on Prediction of Protein Secondary Structure from CD

✍ Scribed by A. Toumadje; W.C. Johnson

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
200 KB
Volume
211
Category
Article
ISSN
0003-2697

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✦ Synopsis

Increasing the magnitude of a protein CD spectrum obviously increases the magnitude of each predicted secondary structure by the same amount. However, increasing the magnitude of the negative, long-wave-length portion of a protein CD spectrum usually has the opposite effect from increasing the positive, short-wave-length portion. Thus small distortions in the CD spectra of proteins at short wavelength can have a significant effect on the analysis for secondary structure. This measurement error and its effect on the analysis are systematically investigated for 16 proteins of known structure. The results demonstrate that a two-point calibration of a CD instrument is mandatory to avoid serious errors when estimating secondary structure from protein CD spectra.

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