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Effects of rat platelet extracts and human platelet-derived growth factor on megakaryocytopoiesis in vitro

✍ Scribed by Ruhi Uyar; Dr. George W. Cooper; Xiao Ping Hou

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
702 KB
Volume
39
Category
Article
ISSN
0022-4790

No coin nor oath required. For personal study only.

✦ Synopsis

An improved plasma clot culture system was developed for the culture of rat megakaryocyte colonies in vitro. Greater stimulation of megakaryocyte colony growth was provided when both serum and plasma were added to the culture medium than when plasma was added alone. Freeze-thawed extracts prepared from 900 x lo6 platelets/ml saline and also 9 ng/ml of human platelet-derived growth factor (PDGF) caused maximum increases over the controls of 7 1 % and 42 % , respectively, in the numbers of megakaryocyte colonies. The increase in the numbers of megakaryocyte colonies induced by platelet extracts and by PDGF implied that substance(s) released by platelets might play an important role in the control of megakaryocytopoiesis.

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