Effects of radiolabelled monoclonal antibody infusion on blood leukocytes in cancer patients

✍ Scribed by Daila S. Gridley; James M. Slater; Dwight R. Stickney

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 580 KB
• Volume: 4
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860040110

✦ Synopsis

Abstract

This study was undertaken to investigate the effects of a single infusion of radiolabelled murine monoclonal antibody (MAb) on peripheral blood leukocytes in cancer patients. Eleven patients with disseminated colon cancer, malignant melanoma, or lung adenocarcinoma were infused with ^111^In‐labelled anti‐ZCE 025, anti‐p97 type 96 ^9^, or LA 20207 MAb, respectively. Blood samples were obtained before infusion, immediately after infusion (1 hr), and at 4 and 7 days postinfusion. Flow cytometry analysis of CD3^+^, CD4^+^, CD8^+^, CD16^+^, and CD19^+^ lymphocytes showed increasing CD4:CD8 ratios in seven patients after infusion. This phenomenon was not restricted to antibody subclass or to type of cancer. Two of the remaining patients exhibited a marked post‐infusion increase in CD8^+^ cells. In all three patients with malignant melanoma, decreasing levels of CD16' lymphocytes were noted after infusion and natural killer cell cytotoxicity showed fluctuations which paralleled the changes in the CD16^+^ subpopulation. Oxygen radical production by phagocytic cells was markedly affected in three subjects. These results suggest that a single infusion of radiolabelled murine MAb may alter the balance of critical lymphocyte subpopulations and modulate other leukocyte responses in cancer patients.