✦ LIBER ✦

Effects of protein kinase inhibitors on regeneration in vitro of adult frog sciatic sensory axons

✍ Scribed by P. A. R. Ekström; H. Bergstrand; A. Edström

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 844 KB
Volume: 31
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490310308

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✦ Synopsis

The effects of protein kinase inhibitors on regeneration in vitro of adult frog sciatic sensory axons were tested. Regeneration of crush-injured nerves for 8 days in serum-free medium was inhibited by staurosporine (100 nM) and H-7 (100 pM), which are both known to inhibit protein kinase C. With the use of a compartmented culture system it could be shown that H-7 exerted both local (outgrowth region) and central (ganglia) effects, the latter being more pronounced. The local effects could be due to reduction of Schwann cell proliferation by H-7. Immunohistochemistry demonstrated the presence of protein kinase C in neuronal cell bodies but not in axonal processes. Proliferation of Schwann cells was accompanied by increased protein kinase C immunoreactivity at the site of injury. H-7 caused a selective inhibition in the incorporation of radioactive phosphate into one 74 kDa protein of both ganglia and nerve but also a more general decrease in protein labelling. The results show that protein phosphorylations, possibly mediated by protein kinase C, are involved in regeneration-related mechanisms operating at both local and central levels in the adult frog sciatic sensory axons.

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