Effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts

✍ Scribed by Carl Rathus; Robert Bower; Robert G. Birch

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 454 KB
• Volume: 23
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00019308

✦ Synopsis

Various chimaeric promoter regions coupled to the uidA fi-glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts. Multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial Emu promoter relative to the CaMV 35S promoter. The relative expression strengths of promoters varied substantially between the species. Sugar-cane also differed in some respects from previously tested species in the family Poaceae. For example, in sugar-cane the nopaline synthase and CaMV 35S promoters were of equivalent strength, and insertion ofAdhl intron 1 into the 5' transcribed region decreased expression strength.