Effects of post-treatment with 6-Dimethylaminopurine (6-DMAP) on ethanol activation of mouse oocytes at different ages
✍ Scribed by Lan, Guo-Cheng ;Ma, Suo-Feng ;Wang, Zi-Yu ;Luo, Ming-Jiu ;Chang, Zhong-Le ;Tan, Jing-He
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 95 KB
- Volume
- 301A
- Category
- Article
- ISSN
- 0022-104X
- DOI
- 10.1002/jez.a.62
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✦ Synopsis
Abstract
To study the effect of post‐treatment with 6‐Dimethylaminopurine (6‐DMAP) on oocyte activation and development, mouse oocytes collected at different times post human chorion gonadotropin (hCG) injection were incubated in 6‐DMAP‐containing Chatot‐Ziomek‐Bavister (CZB) medium for different periods after ethanol exposure, and activation and development were observed. When oocytes were cultured in 6‐DMAP without prior ethanol exposure, the highest activation rate was only 40%. Incubation in 6‐DMAP for 6 h following ethanol exposure significantly (P<0.05) increased the activation rate in oocytes recovered 15 and 18 h post hCG, but this effect was not significant in the 21 h oocytes. When oocytes were incubated in 6‐DMAP for 1 h at different time points after ethanol, a 6‐DMAP susceptible temporal window was found to be located from the second to the fifth h in the 18 h oocytes and from the fourth to the fifth h in the 15 h oocytes, and within the window, the duration of 6‐DMAP incubation can be reduced to 0.5 h with more than 80% activation. With the 13 h oocytes, however, 6‐DMAP‐incubation can only be shortened to 3 h and no specific temporal window was identified. Oocytes that were incubated in 6‐DMAP for 1 or 2 h after ethanol exposure developed to morula/blastocyst stages at significantly (P<0.05) higher rates than those incubated in 6‐DMAP for 6 h. Our results suggested that (i) long duration of 6‐DMAP incubation impaired the development of mouse parthenogenotes; (ii) the effect of 6‐DMAP alone was limited without prior ethanol exposure; (iii) the egg age affected both the timing of 6‐DMAP susceptibility and the duration of exposure required to obtain a maximal activating effect; (iv) the most effective activating protocols varied for oocytes of different ages. J. Exp. Zool. 301A:837–843, 2004. © 2004 Wiley‐Liss, Inc.