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Effects of Polyols and Organic Solvents on Thermostability of Lipase

✍ Scribed by Michiaki Matsumoto; Koji Kida; Kazuo Kondo

Publisher
Wiley (John Wiley & Sons)
Year
1997
Tongue
English
Weight
348 KB
Volume
70
Category
Article
ISSN
0268-2575

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✦ Synopsis

The e †ects of polyols and organic solvents on the thermostability of lipase were studied. The thermodeactivation process of lipase obeyed a series deactivation model and four kinetic parameters were determined. The rate constant of the Ðrst deactivation process was found to be a key one. All the polyols used had a positive e †ect for stabilization of lipase and the extent of e †ect increased with polyol concentration and carbon number of polyol. The e †ect of organic solvents on the thermostability of lipase was investigated by incubating the lipase in the organic solvents before measuring their activities. The behavior of the thermodeactivation depended upon the organic solvents used ; increased activity was observed in some solvents.

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