Effects of phenytoin on cell-mediated immunity

✍ Scribed by Yutaka Okamoto; Keiji Shimizu; Kazuyoshi Tamura; Yasuyoshi Miyao; Masanobu Yamada; Nobuyuki Tsuda; Yutaka Matsui; Heitaro Mogami

• Publisher: Springer-Verlag
• Year: 1988
• Tongue: English
• Weight: 387 KB
• Volume: 26
• Category: Article
• ISSN: 0340-7004
• DOI: 10.1007/bf00205612

✦ Synopsis

The effects of phenytoin on cellular immunity were examined in murine models. Fresh splenocytes were obtained from mice which had received 1 mg/day of phenytoin i.p. for 28 days. The serum concentration of phenytoin in these animals was 10-20 micrograms/ml. The proliferative response of splenocytes to mitogens was assessed by 3H-thymidine incorporation. The cytotoxic activities of cells such as natural killer (NK) cells, cytotoxic T lymphocytes (CTL), and lymphokine-activated killer (LAK) cells were estimated by a 4-h 51Cr release assay. The 3H-thymidine incorporation of splenocytes was reduced significantly (P less than 0.01) in phenytoin-treated mice. The NK and CTL activities of splenocytes from phenytoin-treated mice were significantly suppressed. However, the LAK activity of phenytoin-treated mice was equal to that of control mice.