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Effects of partial hepatectomy on hepatic insulinlike growth factor binding protein-1 expression

✍ Scribed by Aziz Ghahary; Dr. Gerald Y. Minuk; Jiangming Luo; Tony Gauthier; Liam J. Murphy

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 845 KB
Volume: 15
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840150625

No coin nor oath required. For personal study only.

✦ Synopsis

Insulinlike growth factor binding proteins modulate the action of the insulinlike growth factors in various bioassays and may regulate the bioavailability of the insulinlike growth factors in uiuo. Because the insulinlike growth factors may influence hepatic regeneration, we have examined the effect of partial hepatectomy on serum insulinlike growth factor binding proteins and on the abundance of insulinlike growth factor binding protein-1 messenger RNA in the liver. All rats were fasted before and after partial hepatectomy or sham operation to avoid the confounding effects of difference in food intake. Using a conventional protocol, 70% of the liver was removed, and groups of four or five rats were killed at different intervals after partial hepatectomy. Sham-operated rats served as controls. Pooled sera from each group of rats were analyzed by ligand blotting with lZsIinsulinlike growth factor-I. Liver RNA from individual rats was analyzed by slot-blot and Northern-blot hybridization. A small decrease in the 39-to 42-kD insulinlike growth factor binding protein was apparent in sera from both the sham-operated and partial hepatectomized rats. In contrast, a dramatic increase (fivefold) in the 29-kD serum insulinlike growth factor binding protein (insulinlike growth factor binding protein-1) was apparent only in the partial hepatectomized rats. Hepatic insulinlike growth factor binding protein-1 messenger RNA abundance was significantly increased (1.99 f 0.18-fold; p < 0.05) at 1 hr, reached a peak of 2.32 f 0.22-fold (p < 0.01) at 3 hr after partial hepatectomy and returned to basal levels over the subsequent 6 to 12 hr. Interestingly, the abundance of insulinlike growth factor binding protein-1 messenger RNA was also significantly increased in the kidneys of partially hepatectomized rats at 3 hr (1.47 f 0.23-fold; p < 0.05), and peaked at 6 hr (2.50 * 0.39-fold; p < 0.05) after partial hepatectomy. No significant

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