Effects of oxygen concentration on x-ray-induced mitotic inhibition in living Chortophaga neuroblasts

✍ Scribed by Gaulden, Mary Esther ;Nix, Marjorie ;Moshman, Jack

• Publisher: Wiley (John Wiley & Sons)
• Year: 1953
• Tongue: English
• Weight: 976 KB
• Volume: 41
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030410306

✦ Synopsis

Irradiatioii of tissues under ailaerobic coiiditioiis was shown by Crabtree and Cramer ( ' 3 3 ) and Mottram ('%a, 11) to result in a reduction of the inhibition of growth caused by radiation in mouse carcinoma and V i c i u roots. These finding 0s were coiifirmed by Read and Tl'olfe ('48) with Viein, and by Hayden and Smith ('49) with barley, the tvork of the former authors indicating that the extent of inhibition was positively correlated with the amount of osygeii present a t irradiation. Studying a specific aspect of growth, ilndei.son ('39) demonstrated that Arbacin eggs irradiated in hydrogen showed less delay in cleavage than those irradiated in air. C'ohen ('39) found that radiation-induced delay in cleavage of Arbacia eggs was reduced when the eggs were irradiated while closely packed in capillary tubes, a condition which most probably led to reduced oxygen tension. These findings clearly show that the effects of radiation on growth are influenced by oxygen.

The present irivcstigatiori was initiated to determine the effects of oxygen concentration on radiation-induced mitotic inhibition in grasshopper neurohlasts (Gaulden and Nix, '50). These cells can be grown in v i t r o , arid the mitotic rate of large iiumbers of them can be obtained with relative ease over a period of time after irradiation. Xoreover, thc abnormalities 'Work performed under Coiitrnct No. LV 7405 Eng 90 f o r the Atoiiiic Energ! Commission.