Effects of ochratoxin A on micronucleus frequenncy in human lymphocytes

✍ Scribed by Dönmez-Altuntaş, Hamiyet ;Hamurcu, Zuhal ;İmamo??lu, Nalan ;Liman, Bilal Cem

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 61 KB
• Volume: 47
• Category: Article
• ISSN: 0027-769X
• DOI: 10.1002/food.200390005

✦ Synopsis

Abstract

Ochratoxin A (OTA( a nephrotoxic and carcinogenic mycotoxin, was investigated to examine its potency to induce micronuclei (MN) in cultured human lymphocytes. Lymphocyte cultures were treated for the last 48 h with OTA at concentrations of 25 μM, 10 μM, 1 μM, 100 nM, 10 nM, 1 nM, and 100 pM and absolute ethanol. At the highest concentration, OTA was found to induce MN in cytokinesis‐blocked lymphocytes (p < 05). The 25 μM OTA concentration also led to a clear decrease in the percentage of binucleated cells, probably due to cytotoxicity. OTA at the other concentrations tested did not induce MN frequency. These results indicate that a high concentration of OTA is genotoxic in cultured human lymphocytes.