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Effects of Numerical Aperture on Contrast in Ordinary Microscopy

✍ Scribed by OSTERBERG, HAROLD; SMITH, LUTHER W.

Book ID
115379749
Publisher
Optical Society of America
Year
1961
Weight
683 KB
Volume
51
Category
Article
ISSN
0030-3941

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## Abstract Multiphoton microscopy is a powerful technique for achieving three‐dimensional submicron imaging in biological specimens. However, specimen optical parameters such as refractive indices and scattering coefficients can result in the loss of image resolution and decreased signal in depth.