Effects of N,N-dimethylformamide and extracellular matrix on transforming growth factor-β binding to a human colon carcinoma cell line

✍ Scribed by Alan E. Levine; Bradley Black; Michael G. Brattain

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 808 KB
• Volume: 138
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041380304

✦ Synopsis

I c x a s 770 3 0

Alterations in the binding of transforming growth factor-p (TGF-P) to the MOSCR human colon carcinoma cell line caused by N,N-dirnethylformamide (DMF) or extrac-rllular matrix (ECM) werc examined. LIMF induced a morc dif'fercntiated phenntypc in the MOStK cells and resulted in a twofold Increase in TCF-p binding to the cells. This was d u c to an increilse in receptor numbcr with no significant dteration in the K , .

The extent of incwased TGF-p binding was dependent on the dose x t d time of c'xposure to DMF. Upon reinoval of DMF, the receptor levcl returned to that of' untreated cell5 within 6 hr. The binding of TGF-pI and TGF-P:! to the cells was increased equally. Despite this increaw in TGF-P binding in thc presence of DMF, the scnsitivity of the> MOSER cells to the growth inhibitory effects of TGt-p WAS unaltered. Growth of thc MOSEK cells on ECM derived from well-differentidted colon cell line increased the TGF-P