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Effects of mutations and glycosylations on STS activity: A site-directed mutagenesis study

✍ Scribed by Chloe Stengel; Simon P. Newman; Joanna M. Day; Helena J. Tutill; Michael J. Reed; Atul Purohit


Book ID
116734251
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
507 KB
Volume
283
Category
Article
ISSN
0303-7207

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## Abstract By directed evolution and subsequent site‐directed mutagenesis, cold‐adapted variants of WF146 protease, a thermophilic subtilase, have been successfully engineered. A four‐amino acid substitution variant RTN29 displayed a sixfold increase in caseinolytic activity in the temperature ran