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Effects of methanol on expression of an anticoagulant hirudin in recombinantHansenula polymorpha

✍ Scribed by M-D Kim; H-A Kang; S-K Rhee; J-H Seo

Book ID
110050883
Publisher
Springer-Verlag
Year
2001
Tongue
English
Weight
126 KB
Volume
27
Category
Article
ISSN
1476-5535

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Effect of soybean oil on the enhanced ex
Effect of soybean oil on the enhanced expression of hirudin gene inHansenula polymorpha
✍ Chul-Ho Kim; Jung-Hoon Sohn; Eui-Sung Choi; Sang-Ki Rhee πŸ“‚ Article πŸ“… 1996 πŸ› Springer Netherlands 🌐 English βš– 415 KB

A heterologous gene from bloodsucking leech for anticoagulant, hirudin, has been expressed in the methylotrophic yeast Hansenula polymorpha. The addition of 1 %(v/v) soybean oil to the medium as a stabilizer enhanced the expression of the hirudin gene in H. polymorpha with MOX promoter. The product