Effects of metal ions on osteoblast-like cell metabolism and differentiation

✍ Scribed by Sun, Zhi Lin ;Wataha, John C. ;Hanks, Carl T.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 329 KB
• Volume: 34
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199701)34:1<29::aid-jbm5>3.0.co;2-p

✦ Synopsis

The objective of this study was to evaluate the effects of cell metabolism at concentrations below cytotoxic levels. For metal ions, which may be released from orthopedic or dental RNA analysis, Al ϩ3 significantly suppressed the expression implants, on osteoblast metabolism and differentiation. ROS of ALP, OCN, and OPN at both cytotoxic and noncytoxic 17/2.8 cells were cultured in F-12 medium for 7 days. Then concentrations. Co ϩ2 specifically suppressed ALP expression Al ϩ3 , Co ϩ2 , Cr ϩ3 , Ni ϩ2 , Ti ϩ4 , and V ϩ3 were added at concentra-at cytotoxic concentrations. Cr ϩ3 and Ni ϩ2 inhibited OCN, tions less than their cytotoxic concentrations. After 3 days, OPN, and ALP gene expression only at cytotoxic concentra-DNA synthesis, succinate dehydrogenase activity, alkaline tions. For Ti ϩ4 and V ϩ3 ions, gene expression at cytotoxic phosphatase (ALP) activity, and culture calcification were levels was not significantly affected as compared with the assessed. Northern blots were performed for ALP, osteocal-effects at noncytotoxic level. These results show that metal cin (OCN), and osteopontin (OPN) mRNA transcription. The ions may alter osteoblast behavior even at subtoxic concendata indicated that Cr ϩ3 and Al ϩ3 had few inhibitory effects trations, but do not always affect the expression of all genes similarly. ᭧ 1997 John Wiley & Sons, Inc. on ROS cell metabolism below their cytotoxic concentrations, Ni ϩ2 , Co ϩ2 , Ti ϩ4 , and V ϩ3 affected all these parameters of ROS