Effects of low-level laser therapy (LLLT) on the nuclear factor (NF)-κB signaling pathway in traumatized muscle
✍ Scribed by Carem Fetter Rizzi; José Luis Mauriz; Daniela Sousa Freitas Corrêa; Andréa Janz Moreira; Claudio Galeano Zettler; Lidiane Isabel Filippin; Norma Possa Marroni; Javier González-Gallego
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 316 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background and Objective
To investigate the effects of low‐level laser therapy (LLLT) on nuclear factor kappa B (NF‐κB) activation and inducible nitric oxide synthase (iNOS) expression in an experimental model of muscle trauma.
Study Design/Materials and Methods
Injury to the gastrocnemius muscle in the rat was produced by a single impact blunt trauma. A low‐level galium arsenide (Ga–As) laser (904 nm, 45 mW, and 5 J/cm^2^) was applied for 35 seconds duration, continuously.
Results
Histological abnormalities with increase in collagen concentration, and oxidative stress were observed after trauma. This was accompanied by activation of NF‐κB and upregulation of iNOS expression, whereas protein concentration of IκBα decreased. These effects were blocked by LLLT.
Conclusion
LLLT reduced the inflammatory response induced by trauma and was able to block the effects of reactive oxygen species (ROS) release and the activation of NF‐κB. The associated reduction of iNOS overexpression and collagen production suggest that the NF‐κB pathway may be a signaling route involved in the pathogenesis of muscle trauma. Lasers Surg. Med. © 2006 Wiley‐Liss, Inc.