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Effects of low-energy gallium-aluminum-arsenide laser irradiation on cultured fibroblasts and keratinocytes

✍ Scribed by Pogrel, M. Anthony; Chen, Ji Wei; Zhang, Ken

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
201 KB
Volume
20
Category
Article
ISSN
0196-8092

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✦ Synopsis

Background and objective:

To assess whether the gallium-aluminum-arsenide low energy laser will increase cell proliferation, cell attachment, or cell migration in cultured fibroblasts and keratinocyte models.

Study design/materials and methods:

Monolayer cultures of fibroblasts and keratinocytes were subjected to gallium-aluminum-arsenide laser irradiation at varying power densities for varying time intervals. cell proliferation was assessed by absorbent spectrophotometry while cell adhesion was assessed by a microcolorimetric assay for cells attached to bovine dermis collagen. cell migration was assessed through a filter utilizing high power microscopic fields.

Results:

There were no differences in cell proliferation, adhesion, or migration in either the fibroblasts or keratinocyte culture treated with the gallium-aluminum-arsenide laser at any power density or time compared with nontreated controls.

Conclusion:

The gallium-aluminum-arsenide laser, when utilized at powers 5-100 milliwatts and times of between 10-120 seconds has no biostimulatory effects on fibroblasts or keratinocyte cultures as assessed by cell proliferation, adhesion, or migration.

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