Effects of lobenzarit disodium on human endothelial cells. Lobenzarit disodium inhibits proliferative response, HLA–DR antigen expression, and T cell adherence toward endothelial cells
✍ Scribed by Atsushi Kawakami; Katsumi Eguchi; Yukitaka Ueki; Kiyoshi Migita; Hiroaki Ida; Hideto Nakao; Akihiko Kurata; Takaaki Fukuda; Shigenobu Nagataki; Tadayuki Ishimaru; Kenichi Kurouji; Nagatoshi Fujita
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 752 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
The therapeutic action of lobenzarit disodium (CCA) on the function of endothelial cells (EC) isolated from human umbilical cord veins was investigated. CCA suppressed 3H-thymidine incorporation into EC in a dose-dependent manner. Significant inhibition was detected at a concentration of 50 pglml. The expression of HLA-DR antigen on the surface of EC was increased when EC were cultured with recombinant interferon-y (rIFNy). Treatment of EC with either IFNy or interleukin-1 enhanced the adhesion of T cells to EC. The kinetics of HLA-DR antigen expression by EC cultured with IFNy was different from the kinetics of T cell-EC adhesion, however. Neither anti-HLA-DR nor anti-HLA-ABC monoclonal antibody inhibited T cell bind-From the