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Effects of liposome-mediated gene transfer of VEGF in ischemic rat gracilis muscle

✍ Scribed by Michael W. Neumeister; Yao-Hua Song; Arian Mowlavi; Hans Suchy; Aruna Mathur

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
126 KB
Volume
21
Category
Article
ISSN
0738-1085

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✦ Synopsis

Abstract

The purpose of the current study was to determine the effects of vascular endothelial growth factor (VEGF) on muscle flap survival and vascularity in a rat gracilis ischemia‐reperfusion model. A total of 12 adult male Wistar rats were divided into two groups (n = 6). The experimental group received the plasmid encoding VEGF~165~ cDNA plus lipofectamine (cationic liposome) injected directly to the gracilis muscle following 4 h of ischemia. The control group received lipofectamine only. The viability and vascularity of the flaps were evaluated after 7 days of reperfusion. The data demonstrated that the VEGF plasmid‐ and lipofectamine‐treated muscle flaps had significantly greater total survival and capillary count 7 days after reperfusion compared with the flaps treated only with lipofectamine. These results indicate that VEGF exerts a protective effect on ischemic skeletal muscle flaps. © 2001 Wiley‐Liss, Inc. MICROSURGERY 21:58–62 2001

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