Effects of L-proline and post-plating temperature treatment on Maize (Zea maysL.) anther culture
✍ Scribed by B. Büter; J. E. Schmid; P. Stamp
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 533 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
Comparison of different post-plating temperature regimes with a control treatment (27 ~ C) revealed that a short-term cold (8/14~ days or 14~ days) as well as a heat treatment (30~ days) increased the production of embryro-likestructures (ELS) from cultured maize anthers. The beneficial effects of short-term cold treatments were magnified 2-3 times when L-proline (PROL) was added to the induction medium (125 -500 mg/L). In the best treatment (14~ days, 125 mg/L L-proline) one genotype produced 143.5 ELS/100 anthers. Anthers subjected to high temperature (30~ days, 30~ days, 30~ days) generally showed a lower response than did cold treated anthers, although genotypic differences were observed. Regeneration frequency did not appear to be affected by the presence of L-proline in the induction medium.