Effects of kinetin, paclobutrazol and their interactions on the microtuberization of potato stem segments culturedin vitroin the light
✍ Scribed by I. Šimko
- Publisher
- Springer Netherlands
- Year
- 1993
- Tongue
- English
- Weight
- 388 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0167-6903
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✦ Synopsis
Single-nodal cuttings of Solanum tuberosum (four cultivars) and Solanum chacoense were induced to produce in vitro microtubers on Murashige & Skoog (MS) medium supplemented with 8 g 1 -' sucrose and various concentrations of kinetin and paclobutrazol . The cultures were kept 10 days in darkness and then transferred to a 14 h daylength with 100 yE m-2 sec -' light intensity at 21 °C . Kinetin (2 .5 mg 1 -') had no significant influence on tuber formation . However, its addition together with paclobutrazol (0 .001 mg 1 -') significantly enhanced tuberization . Paclobutrazol alone stimulated early tuber initiation and inhibited stem growth . Despite some genotype x treatment interactions, all genotypes (from very early to late and wild type) formed the maximum proportion of explants bearing microtubers on the media containing both plant growth regulators .