Effects of interleukin-5 on acute myeloid leukemias

✍ Scribed by Michael A. Baumann; Cassandra C. Paul; Michael J. Grace

• Book ID: 102699408
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 597 KB
• Volume: 39
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/ajh.2830390407

✦ Synopsis

Because of the known activity of interleukind (IL-5) as a growth factor for human eosinophils, we performed studies to ascertain whether M4Eo acute leukemia cells might be capable of responding to IL-5. Surprisingly, short term incubation of freshly isolated M4EO blasts with rhlL-5 induced differentiation of the cells to macrophages. To determine whether other variants of acute myelogenous leukemia (AML) might also be capable of responding to IL-5, we studied cells from four additional unselected cases and found that one responded to IL-5 by vigorous proliferation. Using biotinylated rhlL-5, second labelling with streptavidin-fluorescein isothiocyanate (FITC) and flow cytometric analysis, specific binding of IL-5 to both responsive leukemias was demonstrated, suggesting the presence of specific IL-5 receptors. Additional study will be needed to assess the frequency of IL-5 responsiveness in AML and to determine the relationship of these phenomena to the physiologic role of IL-5 in the regulation of hematopoiesis.