Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals

✍ Scribed by Jacques Bernier; Michel Fournier; Sylvain De Guise; Daniel Martineau; Pierre Beland

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 148 KB
• Volume: 15
• Category: Article
• ISSN: 0730-7268
• DOI: 10.1002/etc.5620150815

✦ Synopsis

The effects of in vitro exposure of beluga whale splenocytes and thymocytes to different concentrations of mercury chloride (HgCl 2 ), cadmium chloride (CdCl 2 ), and lead chloride (PbCl 2 ) were evaluated. The cells were cultured for 66 h with either concanavalin A (Con-A), phytohemagglutinin-P, or without mitogen, after which percentage of cell death and proliferation were evaluated. Increased percentage of cell death was observed in Con-A-stimulated thymocytes cultured with HgCl 2 , while the viability of splenocytes was not affected by exposure to metals. Decreased splenocyte and thymocyte proliferation was observed with the highest concentration of HgCl 2 and CdCl 2 (10 Ϫ5 M), while lower concentrations of these metals (10 Ϫ6 and 10 Ϫ7 M) as well as all the different concentrations of PbCl 2 (10 Ϫ4 , 10 Ϫ5 , and 10 Ϫ6 M) did not significantly influence cell proliferation. Concentrations of metals that were found to affect the proliferation of beluga lymphocytes are similar to those found in the liver of beluga whales from wild populations.