Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells

✍ Scribed by Hidetsugu Tsujigiwa; Hitoshi Nagatsuka; Mehmet Gunduz; Andrea Rodriguez; Rosario S. Rivera; Racquel Z. LeGeros; Miho Inoue; Noriyuki Nagai

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 170 KB
• Volume: 75A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.30416

✦ Synopsis

Abstract

The use of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP‐2. However, the conventionally used lyophilized rhBMP‐2/collagen mixture does not necessarily give adequate bone‐induction effect. In the present study, we examined the effect of immobilizing rhBMP‐2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP‐2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP‐2 immobilized on succinylated atelocollagen in augmenting cellular activity. © Wiley Periodicals, Inc. J Biomed Mater Res, 2005