Effects of hysterectomy on bone in intact rats, ovariectomized rats, and ovariectomized rats treated with estrogen

✍ Scribed by Dr. Ailsa Goulding; E. Gold; N. J. Lewis-Barned

• Publisher: American Society for Bone and Mineral Research
• Year: 2009
• Tongue: English
• Weight: 555 KB
• Volume: 11
• Category: Article
• ISSN: 0884-0431
• DOI: 10.1002/jbmr.5650110715

✦ Synopsis

To determine whether the uterus plays any role in mediating the ability of estrogen to conserve bone in the rat, eight groups of animals (n = 8) with their skeletons labeled with 45Ca were studied. Rats were ovariectomized (OVX), hysterectomized (Hyst), or given sham operations (Sham) and then pair-fed a low-hydroyproline casein diet for 4 weeks. The groups were treated orally with 17P-estradiol (E,) or vehicle, and serial measurements of biochemical markers of bone breakdown were made in weeks 1, 2, and 4. The femur density was measured by dual-energy X-ray absorptiometry (DXA), and skeletal calcium and 45Ca content were determined chemically. Final total body calcium values (mg) in the eight treatment groups were (means -C SD): Sham, 2573 2 179;