Effects of high ammonium concentrations on growth and anthocyanin formation in grape (Vitis viniferaL.) cell suspension cultured in a production medium

✍ Scribed by Chi Bao Do; François Cormier

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 434 KB
• Volume: 27
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041286

✦ Synopsis

Cultivating Vitis vinifera cell suspensions in a production medium which is characterized by high sucrose and low nitrate concentrations (132 mM and 6.25 mM respectively) repressed growth but enhanced the intracellular accumulation of anthocyanins, especially peonidin 3-glucoside. Increasing the ammonium concentration of the production medium from 2 to 8-16mM increased growth and decreased the accumulation of anthocyanins and peonidin 3-glucoside specifically. Instead, peonidin 3-p-coumaroylglucoside accumulated. At 24 mM ammonium concentration, growth was inhibited and accumulation of peonidin 3-p-coumaroylglucoside was significant (p < 0.05) and represented 42% of total anthocyanins after 12 days of culture compared with 19% in the production medium with 2 mM ammonium.