Effects of hemin on rat liver cyclic AMP-dependent protein kinases in cell extracts and intact hepatocytes
β Scribed by Carolyn D. Scott; Bruce E. Kemp; Anthony M. Edwards
- Book ID
- 119120137
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 603 KB
- Volume
- 847
- Category
- Article
- ISSN
- 0167-4889
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Extracts obtained from rat hepatocytes incubated with saline, glucagon or insulin were electrophoresed on polyacrylamide gels and then assayed for cyclic (3H)AMP binding capacity. Analysis of the binding patterns demonstrated that glucagon dissociated a holoenzyme of cyclic AMP-dependent protein kin
Intact S49 mouse lymphoma cells were used as a model system to study the effects of cyclic AMP (CAMP) and its analogs on the phosphorylation of regulatory (R) subunit of type I CAMP-dependent protein kinase. Phosphorylation of R subunit was negligible in mutants deficient in adenylate cyclase; low l