Effects of granulocytes on human neuroblastoma cells measured by chemiluminescence and chromium-51 release assay
✍ Scribed by Bruchelt, Gernot ;Handgretinger, Rupert ;Kimmig, Astrid ;Goeke, Barbara ;Siedner, Ruben ;Reisfeld, Ralph A. ;Niethammer, Dietrich ;Treuner, Jörn
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 272 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
We investigated whether polymorphonuclear leukocytes (PMN) are able t o kill human neuroblastoma cells either directly or if coated with antibody MAb 14.18 that recognizes ganglioside GD2 present on the cell surface of most neuroblastoma cells. Neuroblastorna cells could not be destroyed directly, whereas in the antibody-dependent reaction (ADCC-reaction) they were easily eliminated. In order t o answer the question whether reacitve oxygen intermediates are involved in this process, chemiluminescence measurements were performed. Compared t o the signals that could be measured using opsonized zymosan as stimulus, only weak CL-signals could be registered during the ADCC reaction.
Pretreatment of PMN with granulocyte-macrophage colony stimulating factor (GM-CSF) enhanced the CL-signals, catalase and SOD reduced it; however, cell killing was only slightly influenced in the presence of catalase and superoxide dismutase. These data suggested that reactive oxygen compounds do not play a prominent role in the killing process. Oefinitive evidence for this suggestion could be obtained using PMN from a patient with chronic granulomatous disease (CGD): MAb 14.18 coated neuroblastoma cells could be killed effectively, but no CL-signal could be registered, either in the AOCC-reaction or using opsonized zymosan as stimulus.