𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B and signal transducers and activators of transcription in TNBS-induced colitis

✍ Scribed by Nelson A. Kretzmann; Henrique Fillmann; José L. Mauriz; Claudio Augusto Marroni; Norma Marroni; Javier González-Gallego; María J. Tuñón

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
545 KB
Volume
14
Category
Article
ISSN
1078-0998

No coin nor oath required. For personal study only.

✦ Synopsis

Background:

We investigated the effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B (NF-B) and signal transducers and activators of transcription (STAT) in a rat model of experimental colitis.

Methods:

Colitis was induced in male Wistar rats by intracolonic administration of 30 mg of 2,4,6-trinitrobenzene sulfonic acid (TNBS). Glutamine (25 mg/kg) was given by rectal route daily for 7 days.

Results: Glutamine significantly reduced gross damage and histopathological scores and prevented the decrease of anal pressure and the elevated myeloperoxidase activity observed in the colon of animals receiving TNBS. TNBS administration induced a marked increase of vascular cell adhesion molecule (VCAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) protein levels. These inflammatory events were associated with increased protein level of NF-B p50 and p65 subunits in the nucleus and significant phosphorylation/degradation of the inhibitor IB␣. Protein levels of the phosphorylated forms of STAT1, STAT5, and Akt were elevated in animals with colonic damage. All these effects were inhibited by administration of glutamine. Increases in the cytosolic concentration of TBARS and hydroperoxide-initiated chemiluminescence, markers of oxidative stress, and levels of tumor necrosis factor ␣ (TNF␣) and interferon ␥ (IFN␥) were significantly inhibited at 48 hours of TNBS instillation in glutamine-treated animals.

Conclusions: Inhibition of the expression of proinflammatory mediators that are regulated by the NF-B and STAT signaling pathways contribute to the therapeutical effect of glutamine in the TNBS model of experimental colitis. These effects may be brought about by inhibition of oxidative stress and reduced expression of proinflammatory cytokines.

📜 SIMILAR VOLUMES

Receptor Activator of Nuclear Factor Kap
Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) Modulates the Expression of Genes Involved in Apoptosis and Cell Cycle in Human Osteoclasts
✍ Erika Rimondi; Marina Zweyer; Enrico Ricci; Roberto Fadda; Paola Secchiero 📂 Article 📅 2007 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 307 KB 👁 2 views

## Abstract It has been clearly established that receptor activator of nuclear factor kappa B ligand (RANKL) is a key cytokine involved in the differentiation of osteoclastic precursors of the monocytic/macrophagic lineage. However, relatively little information is available on the ability of RANKL

Sp1 and Sp3 regulate the basal transcrip
Sp1 and Sp3 regulate the basal transcription of receptor activator of nuclear factor kappa B ligand gene in osteoblasts and bone marrow stromal cells
✍ Jianzhong Liu; Hongmei Yang; Wei Liu; Xuemei Cao; Xu Feng 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 310 KB

## Abstract Receptor activator of nuclear factor kappa B ligand (RANKL), a potent regulator of osteoclast formation and function, is expressed by osteoblasts and bone marrow stromal cells. However, the molecular mechanism underlying RANKL expression in osteoblast/stromal cells remains largely uncle

Discordant effects of activator protein-
Discordant effects of activator protein-1 transcription factor on gene regulation, invasion, and metastasis in spontaneous, radiation-induced, and fos-induced osteosarcomas
✍ Brigitte Rupp; Ursula Lorenz; Jörg Schmidt; Anne Katrin Werenskiold 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 237 KB 👁 1 views

Bone cells are a prime target for the biological function of the fos/jun (activating protein-1 (AP-1)) transcription factor complex. Deregulated expression of c-fos or v-fos in bone cells induces tumorigenicity and the formation of non-metastatic osteosarcomas. In contrast, fos oncogenes transform f

Inhibitory effects of the standardized e
Inhibitory effects of the standardized extract (DA-9601) of Artemisia asiatica Nakai on phorbol ester–induced ornithine decarboxylase activity, papilloma formation, cyclooxygenase-2 expression, inducible nitric oxide synthase expression and nuclear transcription factor κB activation in mouse skin
✍ Hyo-Joung Seo; Kwang-Kyun Park; Seong Su Han; Won-Yoon Chung; Mi-Won Son; Won-Ba 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 636 KB

## Abstract __Artemisia asiatica__ Nakai has been used in traditional Asian medicine for the treatment of inflammatory and other disorders. Previous studies have revealed that the formulated ethanol extract (DA‐9601) of __A. asiatica__ has pronounced antioxidative and antiinflammatory activities an