Quantitative Reverse Transcriptase-Polym
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K.C. Wadhwani; R. Fukuyama; T. Giordano; S.I. Rapoport; K. Chandrasekaran
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Article
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1993
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Elsevier Science
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English
⚖ 885 KB
We investigated the usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify glucose transporter 1 (GLUT1) mRNA in cerebral microvessels. The technique was validated using an in vitro-transcribed RNA fragment (riboprobe) of partial \(3^{\prime}\) noncoding sequence of rat b