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Effects of ethanol ingestion on glucose transporter-1 protein and MRNA levels in rat brain

✍ Scribed by Sant P. Singh; Kalkunte S. Srivenugopal; Xiao-Hua Yuan; Fang Jiang; Ann K. Snyder


Book ID
118346247
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
806 KB
Volume
53
Category
Article
ISSN
0024-3205

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We investigated the usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify glucose transporter 1 (GLUT1) mRNA in cerebral microvessels. The technique was validated using an in vitro-transcribed RNA fragment (riboprobe) of partial \(3^{\prime}\) noncoding sequence of rat b