Effects of elevated temperature and mobile phase composition on a novel C18 silica column

✍ Scribed by J. Andreas Lippert; Todd M. Johnson; Jarem B. Lloyd; Jared P. Smith; Bryce T. Johnson; Jason Furlow; Angela Proctor; Stephanie J. Marin

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 853 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600525

✦ Synopsis

Abstract

A novel polydentate C~18~ silica column was evaluated at an elevated temperature under acidic, basic, and neutral mobile phase conditions using ACN and methanol as the mobile phase organic modifier. The temperature range was 40–200°C. The mobile phase compositions were from 0 to 80% organic–aqueous v/v and the mobile phase pH levels were between 2 and 12. The maximum operating temperature of the column was affected by the amount and type of organic modifier used in the mobile phase. Under neutral conditions, the column showed good column thermal stability at temperatures ranging between 120 and 200°C in methanol–water and ACN–water solvent systems. At pH 2 and 3, the column performed well up to about 160°C at two fixed ACN–buffer compositions. Under basic conditions at elevated temperatures, the column material deteriorated more quickly, but still remained stable up to 100°C at pH 9 and 60°C at pH 10. The results of this study indicate that this novel C~18~ silica‐based column represents a significant advancement in RPLC column technology with enhanced thermal and pH stability when compared to traditional bonded phase silica columns.